C-Reactive Protein Induces Signaling Through FcγRIIa on HL-60 Granulocytes

C-Reactive Protein Induces Signaling

Phospholipase C activates protein kinase C and induces monocytic differentiation of HL-60 cells.

Phospholipase C (PLC)-mediated hydrolysis of membrane phospholipids results in the production of diacylglycerol, inositol phosphates, and choline metabolites. Inositol triphosphate increases calcium levels, while diacylglycerol activates protein kinase C. The present studies demonstrate that exogenous PLC generates inositol phosphates, releases choline metabolites, and activates protein kinase ...

Bryostatin I Activates Protein Kinase C and Induces Monocytic Differentiation of HL-60 Cells

Phorbol esters induce the human HL-60 promyelocytic cell line to differentiate along a monocytic pathway. This induction of differentiation may involve phorbol esterinduced activation of the phospholipidand calcium-dependent protein kinase C. Bryostatin 1 #{149} a macrocyclic lactone. has been shown to compete with phorbol esters for binding to protein kinase C. We have confirmed that bryostati...

Bryostatin 1 activates protein kinase C and induces monocytic differentiation of HL-60 cells.

Phorbol esters induce the human HL-60 promyelocytic cell line to differentiate along a monocytic pathway. This induction of differentiation may involve phorbol ester-induced activation of the phospholipid- and calcium-dependent protein kinase C. Bryostatin 1, a macrocyclic lactone, has been shown to compete with phorbol esters for binding to protein kinase C. We have confirmed that bryostatin 1...

The proinflammatory effect of C-reactive protein on human endothelial cells depends on the FcγRIIa genotype.

INTRODUCTION The stimulatory effects of CRP (C-reactive protein) on endothelial cells are mainly mediated via FcγRIIa. This receptor exists in two different allotypes bearing either arginine (R131) or histidine (H131) at the extracellular amino acid position 131 of the mature protein, but only FcγRIIa-R131 displays high avidity for CRP. This study investigated the role of the FcγRIIa genotype i...